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adenylate kinase may result in conversion of ADP into ATP and AMP and thereby confound interpretation from the ADP/ATP specificity experiments. Solving the conundrum of how some twin-specificity archaeal ligases could utilize ATP and NAD+ will depend on capturing crystal structures of these enzymes with Each and every nucleotide bound.
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The movement involves a rigid overall body rotation about the hinge segment connecting the NTase and OB domains (indicated because of the arrow
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(eighteen) have executed a virtual display screen for little molecules that may dock in the N-terminal DBD of HuLig1, a site conserved in all mammalian DNA ligases. They thereby discovered compounds that (i) inhibited nick sealing by human ligases in vitro
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